qPCR assay for the detection of Mycobacterium avium subsp. paratuberculosis DNA in samples from Cattle, Small ruminants.
Details
INgene® q Paratuberculosis (multiplex) is a real-time PCR kit for the detection of PTBC F57 and PTBC IS900 DNA in biological samples from Cattle, Small ruminants. The assay is optimized for samples like faeces, rectal swabs, milk, tissues including intestine, ileocecal valve, lymph nodes and strains/vaccines. The kit consists on a multiplex real-time PCR in one-well format, detecting F57 gene in FAM channel and IS9 gene in Cy5 channel. In addition, the mix includes and Endogenous control (EC) that is amplified in HEX/VIC, allowing identification of false negatives due to poor extraction and/or PCR inhibition.
Main assay benefits are:- Easy to use format, minimun preparation time
- Harmonized thermal profile across most INgene® q kits
- Broadly compatible with available PCR instruments
- Optional quantification with the positive control
- Validated with clinical samples
- Extended shelf life: 18 months
- 100 rxn format
Kit Specifications
SKU
R.10.XPT.K.5/100
Test principle
qPCR
No. of tests
100
Min no. of samples
100
Max no. of samples
100
Detection
Mycobacterium avium subsp. paratuberculosis DNA in samples from Cattle, Small ruminants.
Shelf Life (months)
12
Storage
-18°C
Technology
PCR